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trnK-UUU-rps16

LSC · 4281-5133 · 853 bp

Back to Sycopsis

Pi 0.00469

MarkerSeek score 61.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 4
pi: 0.00469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.2	58.2	940-943 bp	1.0	77.4
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.2	58.2	939-942 bp	1.0	77.4
trnK-UUU-rps16_p3	TCCTAGCCGCACTTAAAAGC	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.3	58.2	955-958 bp	1.0	76.6
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.3	58.2	949-952 bp	1.0	76.5
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GGTTTTTACGGAACTTCGCC	50.0	50.0	58.2	57.7	929-932 bp	1.0	76.4