Hotspot

rps15

SSC · 123487-123759 · 273 bp

Back to Syagrus

Pi 0

MarkerSeek score 19.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GGCTCAGCTCCAGCATAATT	ACGAAATCGGCAGAATCCTT	50.0	45.0	58.0	57.6	980-983 bp	1.0	77.6
rps15_p2	AACACCATGCATTGATGGGT	ACGAAATCGGCAGAATCCTT	45.0	45.0	58.1	57.6	727-730 bp	1.0	77.6
rps15_p3	CCTCTCCACCAATAATGCCC	ACGAAATCGGCAGAATCCTT	55.0	45.0	58.0	57.6	1289-1292 bp	1.0	77.5
rps15_p4	GAAGCTATACGGCTCAGCTC	ACGAAATCGGCAGAATCCTT	55.0	45.0	57.9	57.6	990-993 bp	1.0	77.3
rps15_p5	GAGAAGCTATACGGCTCAGC	ACGAAATCGGCAGAATCCTT	55.0	45.0	57.9	57.6	992-995 bp	1.0	77.3