Hotspot

rpl16

LSC · 81338-82926 · 1589 bp

Back to Syagrus

Pi 0.00157

MarkerSeek score 60.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1589
variable_sites: 5
pi: 0.00157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCGGTTGCTTTTTCATCCTC	AGGTTGAATAAAAATTCGATTGACCA	50.0	30.8	58.0	58.1	1667-1680 bp	1.0	40.9
rpl16_p2	GCGGTTGCTTTTTCATCCTC	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	58.0	56.9	1666-1679 bp	1.0	40.9
rpl16_p3	AAACCTGCGGTTGCTTTTTC	AGGTTGAATAAAAATTCGATTGACCA	45.0	30.8	58.1	58.1	1673-1686 bp	1.0	40.9
rpl16_p4	AAACCTGCGGTTGCTTTTTC	GGTTGAATAAAAATTCGATTGACCA	45.0	32.0	58.1	56.9	1672-1685 bp	1.0	40.9
rpl16_p5	GCGGTTGCTTTTTCATCCTC	AGGTTGAATAAAAATTCGATTGACC	50.0	32.0	58.0	56.6	1667-1680 bp	1.0	40.9