Hotspot

psbC

LSC · 33006-34427 · 1422 bp

Back to Syagrus

Pi 0.000352

MarkerSeek score 33.80

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTTCCCAAGAAATCCGTGCA	TAGCCGAGCCAGAACAGAAA	45.0	50.0	58.0	59.0	1585 bp	1.0	71.7
psbC_p2	TTTCCCAAGAAATCCGTGCA	GCCGAGCCAGAACAGAAAAA	45.0	50.0	58.0	59.0	1583 bp	1.0	71.6
psbC_p3	TTTCCCAAGAAATCCGTGCA	TAGCCGAGCCAGAACAGAA	45.0	52.6	58.0	58.3	1585 bp	1.0	69.3
psbC_p4	GCAGCGGAAGATCCTGAATT	TAGCCGAGCCAGAACAGAAA	50.0	50.0	58.3	59.0	1568 bp	1.0	69.2
psbC_p5	AGGTATTCGTGCTTGGATGG	TAGCCGAGCCAGAACAGAAA	50.0	50.0	57.7	59.0	1512 bp	1.0	69.2