Hotspot

ccsA

SSC · 114356-115324 · 969 bp

Back to Syagrus

Pi 0.00413
MarkerSeek score 51.20
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
969
variable_sites
7
pi
0.00413
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 CCGATTTCAATTCTGTAATGGGA ACTCGAACAAGCAAACTTTCG 39.1 42.9 56.9 57.7 1067 bp 0.75 30.4
ccsA_p2 CCGATTTCAATTCTGTAATGGGA CTCGAACAAGCAAACTTTCGT 39.1 42.9 56.9 57.7 1066 bp 0.75 30.4
ccsA_p3 TCCGATTTCAATTCTGTAATGGG ACTCGAACAAGCAAACTTTCG 39.1 42.9 56.9 57.7 1068 bp 0.75 30.4
ccsA_p4 TCCGATTTCAATTCTGTAATGGG CTCGAACAAGCAAACTTTCGT 39.1 42.9 56.9 57.7 1067 bp 0.75 30.4
ccsA_p5 CCGATTTCAATTCTGTAATGGGAC CTCGAACAAGCAAACTTTCGT 41.7 42.9 58.2 57.7 1066 bp 0.75 30.4