Hotspot

rps15-ycf1

SSC · 123760-124153 · 394 bp

Back to Syagrus

Pi 0.0106

MarkerSeek score 61.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 7
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGAACCCTTGTTTTCT	TGGCAGTCGTTTCAGTATGT	38.1	45.0	55.5	57.2	518-520 bp	1.0	47.1
rps15-ycf1_p2	TCAACAGAACCCTTGTTTTCTTC	TGGCAGTCGTTTCAGTATGT	39.1	45.0	57.4	57.2	518-520 bp	1.0	46.1
rps15-ycf1_p3	CAACAGAACCCTTGTTTTCTTCT	TGGCAGTCGTTTCAGTATGT	39.1	45.0	57.4	57.2	517-519 bp	1.0	46.1
rps15-ycf1_p4	TGAAATTCAACAGAACCCTTGT	TGGCAGTCGTTTCAGTATGT	36.4	45.0	56.4	57.2	524-526 bp	1.0	45.7
rps15-ycf1_p5	TCAACAGAACCCTTGTTTTCTT	TGGCAGTCGTTTCAGTATGT	36.4	45.0	56.3	57.2	518-520 bp	1.0	45.2