Hotspot

rpl22-rps19

LSC · 84182-84323 · 142 bp

Back to Syagrus

Pi 0.0109

MarkerSeek score 63.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 3
pi: 0.0109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	50.0	50.0	58.5	58.0	302-307 bp	1.0	77.9
rpl22-rps19_p2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	50.0	55.0	58.5	57.8	307-312 bp	1.0	76.6
rpl22-rps19_p3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	50.0	50.0	58.5	57.7	303-308 bp	1.0	75.9
rpl22-rps19_p4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	50.0	50.0	58.5	58.5	326-331 bp	1.0	74.3
rpl22-rps19_p5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	50.0	45.0	58.5	58.5	295-300 bp	1.0	74.2