Hotspot

petA-psbJ

LSC · 62682-63616 · 935 bp

Back to Syagrus

Pi 0.00802

MarkerSeek score 55.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 15
pi: 0.00802
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1087-1102 bp	1.0	78.3
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1083-1098 bp	1.0	77.4
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1070-1085 bp	1.0	77.4
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1086-1101 bp	1.0	77.4
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1084-1099 bp	1.0	77.2