Hotspot

trnT-UGU-trnL-UAA

LSC · 45395-46315 · 921 bp

Back to Syagrus

Pi 0.00416

MarkerSeek score 61.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 7
pi: 0.00416
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	AAAGCCAGCTATCGGAATCG	ATCCAATCAAGTCCGTAGCG	50.0	50.0	58.1	57.8	1017-1019 bp	1.0	77.9
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	972-974 bp	1.0	77.9
trnT-UGU-trnL-UAA_p3	AAAGCCAGCTATCGGAATCG	AGGATTTGGCTCAGGATTGC	50.0	50.0	58.1	58.2	1106-1108 bp	1.0	77.8
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGGATTTGGCTCAGGATTGC	55.0	50.0	57.9	58.2	1061-1063 bp	1.0	77.8
trnT-UGU-trnL-UAA_p5	AAAGCCAGCTATCGGAATCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.1	57.7	1014-1016 bp	1.0	77.4