Hotspot

matK-trnK-UUU

LSC · 3130-3947 · 818 bp

Back to Syagrus

Pi 0.00122

MarkerSeek score 60.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 2
pi: 0.00122
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAAAGTGTTGTTGCCGAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.7	58.2	894-899 bp	1.0	76.9
matK-trnK-UUU_p2	AGAAAGTGTTGTTGCCGAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.7	58.2	893-898 bp	1.0	76.9
matK-trnK-UUU_p3	AGAAAGTGTTGTTGCCGAGA	GTCGTGCAGTGCAATTCAAT	45.0	45.0	57.7	57.7	974-979 bp	1.0	75.4
matK-trnK-UUU_p4	AAGTGTTGTTGCCGAGATCT	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.4	58.2	891-896 bp	1.0	75.1
matK-trnK-UUU_p5	AAGTGTTGTTGCCGAGATCT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.4	58.2	890-895 bp	1.0	75.1