Hotspot

rps16

LSC · 5088-6211 · 1124 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1124
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGGGTGTGGATGAGTCCTAT	AAAAGACTGTGTATCGGCCG	55.0	50.0	58.2	58.3	1284 bp	1.0	76.2
rps16_p2	GGGGTGTGGATGAGTCCTAT	CGGCCGAAGCAATGACTATT	55.0	50.0	58.2	58.7	1270 bp	1.0	72.9
rps16_p3	GGGGTGTGGATGAGTCCTAT	CGGCCGAAGCAATGACTAT	55.0	52.6	58.2	58.0	1270 bp	1.0	70.4
rps16_p4	GGGGTGTGGATGAGTCCTAT	GTATCGGCCGAAGCAATGA	55.0	52.6	58.2	58.0	1274 bp	1.0	70.4
rps16_p5	GGGGTGTGGATGAGTCCTAT	TATCGGCCGAAGCAATGAC	55.0	52.6	58.2	58.0	1273 bp	1.0	70.4