Hotspot

psbA

LSC · 286-1347 · 1062 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GCAATACCGACTCTCTTGCA	TGCTTGGGAGTCCCTGATAA	50.0	50.0	58.0	58.0	1178 bp	1.0	79.6
psbA_p2	TGCAAGAGAGTCGGTATTGC	TGCTTGGGAGTCCCTGATAA	50.0	50.0	58.0	58.0	1162 bp	1.0	79.6
psbA_p3	AGCAATACCGACTCTCTTGC	TGCTTGGGAGTCCCTGATAA	50.0	50.0	57.7	58.0	1179 bp	1.0	77.3
psbA_p4	GCAAGAGAGTCGGTATTGCT	TGCTTGGGAGTCCCTGATAA	50.0	50.0	57.7	58.0	1161 bp	1.0	77.3
psbA_p5	TGCAAGAGAGTCGGTATTGC	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.0	58.4	1169 bp	1.0	76.9