Hotspot

atpI

LSC · 15955-16698 · 744 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	ACATTGCCTTGTTCTAAGCT	AGTCGCAAAGAAGATGGTTGA	40.0	42.9	55.2	57.9	854 bp	1.0	48.9
atpI_p2	ACATTGCCTTGTTCTAAGCT	ACAAGTCGCAAAGAAGATGGT	40.0	42.9	55.2	58.1	857 bp	1.0	48.7
atpI_p3	ACATTGCCTTGTTCTAAGCT	GACAAGTCGCAAAGAAGATGG	40.0	47.6	55.2	57.5	858 bp	1.0	46.2
atpI_p4	ACATTGCCTTGTTCTAAGCT	GTCGCAAAGAAGATGGTTGA	40.0	45.0	55.2	56.4	853 bp	1.0	44.8
atpI_p5	ACATTGCCTTGTTCTAAGCT	AGTCGCAAAGAAGATGGTTG	40.0	45.0	55.2	56.4	854 bp	1.0	44.8