Hotspot

atpH-atpI

LSC · 14767-15954 · 1188 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1309 bp	1.0	78.7
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1331 bp	1.0	78.0
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	1283 bp	1.0	77.6
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.2	57.9	1335 bp	1.0	77.4
atpH-atpI_p5	CAATAGAAGCAAGCCCGACA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.3	57.9	1310 bp	1.0	76.8