Hotspot

trnS-GCU-trnG-UCC

LSC · 9159-9935 · 777 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TAAGCCACTCAGCCATCTCT	AACTCAAGGACCCCTTAACT	50.0	45.0	58.1	55.6	871 bp	1.0	60.1
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AACTCAAGGACCCCTTAACT	50.0	45.0	58.2	55.6	938 bp	1.0	60.0
trnS-GCU-trnG-UCC_p3	AAGATACCAAGCCATCGACG	AACTCAAGGACCCCTTAACT	50.0	45.0	57.8	55.6	961 bp	1.0	59.4
trnS-GCU-trnG-UCC_p4	TAAGCCACTCAGCCATCTCT	AAACTCAAGGACCCCTTAACT	50.0	42.9	58.1	56.5	872 bp	1.0	58.6
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AAACTCAAGGACCCCTTAACT	50.0	42.9	58.2	56.5	939 bp	1.0	58.4