Hotspot

trnK-UUU-rps16

LSC · 4221-5087 · 867 bp

Back to Swietenia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
867
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGCCGTACTTAAAAGCCGAG GCGCTCAACCTACAGGAAC 50.0 57.9 58.0 58.5 1001 bp 1.0 67.6
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GCGCTCAACCTACAGGAAC 50.0 57.9 58.2 58.5 990 bp 1.0 66.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCGCTCAACCTACAGGAAC 50.0 57.9 58.2 58.5 989 bp 1.0 66.3
trnK-UUU-rps16_p4 GCCGTACTTAAAAGCCGAGT GCGCTCAACCTACAGGAAC 50.0 57.9 58.3 58.5 1000 bp 1.0 65.4
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GCGCTCAACCTACAGGAAC 50.0 57.9 57.5 58.5 976 bp 1.0 64.0