Hotspot

rps16

LSC · 4964-5990 · 1027 bp

Back to Swertia

Pi 0.0394

MarkerSeek score 69.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1027
variable_sites: 144
pi: 0.0394
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGTTGAGCTCCCCTTTCAAG	AAATTCTAGAAGAACAAGGGA	55.0	33.3	58.5	52.0	974-1240 bp	0.565217	34.6
rps16_p2	GTTGAGCTCCCCTTTCAAGG	AAATTCTAGAAGAACAAGGGA	55.0	33.3	58.5	52.0	973-1239 bp	0.565217	34.6
rps16_p3	ATATCCTGAACCGTTCCCGT	AAATTCTAGAAGAACAAGGGA	50.0	33.3	58.5	52.0	995-1261 bp	0.565217	34.6
rps16_p4	AATATCCTGAACCGTTCCCG	AAATTCTAGAAGAACAAGGGA	50.0	33.3	57.4	52.0	996-1262 bp	0.565217	34.6
rps16_p5	GTTGAGCTCCCCTTTCAAGG	AAATTCTAGAAGAACAAGGGAT	55.0	31.8	58.5	52.7	973-1239 bp	0.565217	34.6