Hotspot

rpl32-trnL-UAG

SSC · 112414-113334 · 921 bp

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Pi 0.0459

MarkerSeek score 85.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 921
variable_sites: 188
pi: 0.0459
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGTAAATCTCTTTCGACTGGA	TCGAACCGAGATGCTTTAGC	40.9	50.0	56.6	58.1	961-1164 bp	1.0	64.0
rpl32-trnL-UAG_p2	AGGTAAATCTCTTTCGACTGGA	TCCTAAGAGCAGCGTGTCTA	40.9	50.0	56.6	57.9	935-1138 bp	1.0	63.6
rpl32-trnL-UAG_p3	AGGTAAATCTCTTTCGACTGGA	AGCAGCGTGTCTACCAATTT	40.9	45.0	56.6	57.8	928-1131 bp	1.0	63.1
rpl32-trnL-UAG_p4	AGGTAAATCTCTTTCGACTGGA	CCTAAGAGCAGCGTGTCTAC	40.9	55.0	56.6	57.8	934-1137 bp	1.0	63.0
rpl32-trnL-UAG_p5	AGGTAAATCTCTTTCGACTGGA	CTAAGAGCAGCGTGTCTACC	40.9	55.0	56.6	57.8	933-1136 bp	1.0	63.0