Hotspot

ndhF-rpl32

SSC · 111499-112248 · 750 bp

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Pi 0.0456
MarkerSeek score 78.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
750
variable_sites
128
pi
0.0456
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCATATTGATATGTTTGTTGCA TGATGTAGAAATGCGCTTTT 31.8 35.0 53.2 53.9 559-818 bp 1.0 52.0
ndhF-rpl32_p2 CCATATTGATATGTTTGTTGCA TTGATGTAGAAATGCGCTTT 31.8 35.0 53.2 53.9 560-819 bp 1.0 52.0
ndhF-rpl32_p3 CCATATTGATATGTTTGTTGCA TTTGATGTAGAAATGCGCTT 31.8 35.0 53.2 53.9 561-820 bp 1.0 52.0
ndhF-rpl32_p4 CCATATTGATATGTTTGTTGCA TTTTGATGTAGAAATGCGCT 31.8 35.0 53.2 53.9 562-821 bp 1.0 52.0
ndhF-rpl32_p5 CCATATTGATATGTTTGTTGCA TGATGTAGAAATGCGCTTTTT 31.8 33.3 53.2 54.8 559-818 bp 1.0 52.0