Hotspot

ycf3-trnS-GGA

LSC · 44810-45310 · 501 bp

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Pi 0.0334
MarkerSeek score 71.00
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
501
variable_sites
74
pi
0.0334
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCCATTTATTCGTGATCTAGGCA TTCCAATGCTACGCCTTGAA 39.1 45.0 57.5 57.8 438-587 bp 1.0 62.1
ycf3-trnS-GGA_p2 TGGCTACAATTGAAAAGGTCT TTCCAATGCTACGCCTTGAA 38.1 45.0 55.4 57.8 472-621 bp 1.0 61.6
ycf3-trnS-GGA_p3 TGGCTACAATTGAAAAGGTCTT TTCCAATGCTACGCCTTGAA 36.4 45.0 56.2 57.8 472-621 bp 1.0 59.8
ycf3-trnS-GGA_p4 TTGGCTACAATTGAAAAGGTCT TTCCAATGCTACGCCTTGAA 36.4 45.0 56.2 57.8 473-622 bp 1.0 59.8
ycf3-trnS-GGA_p5 TCCATTTATTCGTGATCTAGGCA AGTTCCAATGCTACGCCTTG 39.1 50.0 57.5 58.5 440-589 bp 1.0 59.3