Hotspot

rpoB-trnC-GCA

LSC · 26047-27234 · 1188 bp

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Pi 0.0344

MarkerSeek score 74.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 175
pi: 0.0344
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ACTCCCTCATTTCCATCAACG	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.0	58.1	1154-1401 bp	1.0	83.3
rpoB-trnC-GCA_p2	ACTCCCTCATTTCCATCAACG	CCGAAGAGGGGATTTCAAGG	47.6	55.0	58.0	58.0	1194-1441 bp	0.956522	81.8
rpoB-trnC-GCA_p3	CCTCATTTCCATCAACGAGC	CCGAAGAGGGGATTTCAAGG	50.0	55.0	56.9	58.0	1190-1437 bp	0.956522	80.9
rpoB-trnC-GCA_p4	ACTCCCTCATTTCCATCAACG	TATCAGCCGAAGAGGGGATT	47.6	50.0	58.0	57.9	1200-1447 bp	0.869565	77.9
rpoB-trnC-GCA_p5	ACTCCCTCATTTCCATCAACG	TTATCAGCCGAAGAGGGGAT	47.6	50.0	58.0	57.9	1201-1448 bp	0.869565	77.9