Hotspot

atpA-atpF

LSC · 10937-11005 · 69 bp

Back to Swertia

Pi 0.0458

MarkerSeek score 69.40

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 11
pi: 0.0458
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	GTCGCCTACTTGAAGCACAT	ACATGTTGGGGTCAATAAAAGA	50.0	36.4	58.3	55.9	198-214 bp	1.0	54.6
atpA-atpF_p2	GTCGCCTACTTGAAGCACAT	TGCAAATATTAACATGTTGGGGT	50.0	34.8	58.3	57.0	209-225 bp	0.956522	52.0
atpA-atpF_p3	GTCGCCTACTTGAAGCACAT	AGTGCAAATATTAACATGTTGGGG	50.0	37.5	58.3	58.0	211-227 bp	0.956522	51.9
atpA-atpF_p4	GTCGCCTACTTGAAGCACAT	GCAAATATTAACATGTTGGGGTCA	50.0	37.5	58.3	58.0	208-224 bp	0.956522	51.9
atpA-atpF_p5	GTCGCCTACTTGAAGCACAT	TGCAAATATTAACATGTTGGGGTC	50.0	37.5	58.3	58.0	209-225 bp	0.956522	51.9