Hotspot

trnS-GCU-trnG-UCC

LSC · 7801-8304 · 504 bp

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Pi 0.0357
MarkerSeek score 71.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
504
variable_sites
79
pi
0.0357
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGTCGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 557-701 bp 1.0 63.7
trnS-GCU-trnG-UCC_p2 AATCCGTCGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 556-700 bp 1.0 63.7
trnS-GCU-trnG-UCC_p3 CAATCCGTCGCTTTAGTCCA AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 558-702 bp 1.0 61.8
trnS-GCU-trnG-UCC_p4 AATCCGTCGCTTTAGTCCAC AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 557-701 bp 1.0 61.7
trnS-GCU-trnG-UCC_p5 AGCAATCCGTCGCTTTAGTC ACGAATCACACTTTTACCACT 50.0 38.1 58.4 55.5 559-703 bp 1.0 60.8