Hotspot

psbK-psbI

LSC · 7101-7499 · 399 bp

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Pi 0.0336

MarkerSeek score 70.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 55
pi: 0.0336
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.1	57.4	432-515 bp	1.0	70.9
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	57.9	57.4	427-510 bp	1.0	70.9
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	424-507 bp	1.0	68.8
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	425-508 bp	1.0	68.8
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	426-509 bp	1.0	68.8