Hotspot

rps16-trnQ-UUG

LSC · 5991-6504 · 514 bp

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Pi 0.043

MarkerSeek score 72.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 89
pi: 0.043
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCAAAGGGCGTTTCCTTGT	TGAGTTGAGGGGTTCGTACT	45.0	50.0	58.0	58.0	1573-1995 bp	0.652174	77.1
rps16-trnQ-UUG_p2	ATCAAAGGGCGTTTCCTTGT	AGCAGCTTGCCAAACAAAAG	45.0	45.0	58.0	58.1	1447-1870 bp	0.652174	77.0
rps16-trnQ-UUG_p3	ATCAAAGGGCGTTTCCTTGT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	1452-1875 bp	0.652174	76.9
rps16-trnQ-UUG_p4	AATTTGCTCGACTGGGATCC	TGAGTTGAGGGGTTCGTACT	50.0	50.0	58.0	58.0	1478-1794 bp	0.608696	74.1
rps16-trnQ-UUG_p5	CCAGCTGTAACCAGTCGTAC	TGAGTTGAGGGGTTCGTACT	55.0	50.0	58.0	58.0	1915-2181 bp	0.521739	69.8