Hotspot

trnH-GUG-psbA

LSC · 75-424 · 350 bp

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Pi 0.0881

MarkerSeek score 71.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 350
variable_sites: 101
pi: 0.0881
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CCACTTGGCTACATCCGC	TTCCTCTAGACCTAGCTGCT	61.1	50.0	58.2	56.9	372-439 bp	1.0	65.4
trnH-GUG-psbA_p2	CCACTTGGCTACATCCGC	TTTCCTCTAGACCTAGCTGCT	61.1	47.6	58.2	57.6	373-440 bp	1.0	63.4
trnH-GUG-psbA_p3	CCACTTGGCTACATCCGC	ACCGTGCTAATCTTGGTATGG	61.1	47.6	58.2	57.8	423-490 bp	0.956522	63.2
trnH-GUG-psbA_p4	CCACTTGGCTACATCCGC	CCGTGCTAATCTTGGTATGGA	61.1	47.6	58.2	57.5	422-489 bp	0.956522	61.0
trnH-GUG-psbA_p5	CCACTTGGCTACATCCGC	CTTTCCTCTAGACCTAGCTGC	61.1	52.4	58.2	57.3	374-441 bp	1.0	60.7