Hotspot

ycf3

LSC · 41523-43475 · 1953 bp

Back to Swartzia

Pi 0.496

MarkerSeek score 80.20

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1953
variable_sites: 838
pi: 0.496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GGCCCAGAAATCGAGTGAAT	ATCACGTGAGCAGCCATTAG	50.0	50.0	58.0	58.1	2567 bp	0.5	59.2
ycf3_p2	GGCCCAGAAATCGAGTGAAT	ACACATACAAAGAGACCCGC	50.0	50.0	58.0	57.9	2937 bp	0.5	58.9
ycf3_p3	GGCCCAGAAATCGAGTGAAT	TGGGGGACATACCGGTATTT	50.0	50.0	58.0	58.1	2541 bp	0.5	58.8
ycf3_p4	GGCCCAGAAATCGAGTGAAT	CAAAGAGACCCGCCAACATA	50.0	50.0	58.0	57.9	2930 bp	0.5	58.8
ycf3_p5	GGCCCAGAAATCGAGTGAAT	CATACAAAGAGACCCGCCAA	50.0	50.0	58.0	57.9	2934 bp	0.5	58.8