Hotspot

trnP-UGG-psaJ

LSC · 70570-71097 · 528 bp

Back to Swartzia

Pi 0.0594

MarkerSeek score 81.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 528
variable_sites: 26
pi: 0.0594
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAGCCCCGAACCATAGAGTA	45.0	50.0	57.9	57.8	545-627 bp	1.0	85.3
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	GTACTTACCACTGGTGCCAC	45.0	55.0	57.9	58.2	528-610 bp	1.0	85.2
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	AAGCCCCGAACCATAGAGTA	45.0	50.0	58.2	57.8	546-628 bp	1.0	84.8
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	GTACTTACCACTGGTGCCAC	45.0	55.0	58.2	58.2	529-611 bp	1.0	84.7
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	AAGCCCCGAACCATAGAGTA	45.0	50.0	57.8	57.8	553-635 bp	1.0	84.6