Hotspot

ndhC-trnV-UAC

LSC · 54440-55069 · 630 bp

Back to Swartzia

Pi 0.0727
MarkerSeek score 93.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
630
variable_sites
44
pi
0.0727
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
93.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 773-800 bp 1.0 82.7
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 772-799 bp 1.0 80.0
ndhC-trnV-UAC_p3 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 780-807 bp 1.0 79.8
ndhC-trnV-UAC_p4 TCTCTGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 781-808 bp 1.0 79.6
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 761-788 bp 1.0 78.9