Hotspot

ycf3-psaA

LSC · 43476-44237 · 762 bp

Back to Swartzia

Pi 0.469

MarkerSeek score 79.40

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 283
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	ATGCCACTCAGCCAAAGAAA	50.0	45.0	58.0	58.0	1092 bp	0.5	60.0
ycf3-psaA_p2	CGGGAAACTCTCAAGTACGG	ATGCCACTCAGCCAAAGAAA	55.0	45.0	58.0	58.0	1232 bp	0.5	59.9
ycf3-psaA_p3	GAATTGACTGCTGAGCCGTA	ATGCCACTCAGCCAAAGAAA	50.0	45.0	58.0	58.0	1257 bp	0.5	59.9
ycf3-psaA_p4	TTAAGGTTTTGAGCAGCGGT	ATGCCACTCAGCCAAAGAAA	45.0	45.0	58.0	58.0	1562 bp	0.5	59.8
ycf3-psaA_p5	AGCCCTTACCCCTGGTAATT	CTAGTAGCCCTGCCAAATGG	50.0	55.0	58.0	58.0	1439 bp	0.5	59.7