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trnT-UGU-rps4

LSC · 40008-40401 · 394 bp

Back to Swartzia

Pi 0.462

MarkerSeek score 77.80

Primer available yes

Label trnT-UGU-rps4

Feature Metadata

label_name: trnT-UGU-rps4
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 163
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-rps4_p1	CTCAGAGGTTAGAGCATCGC	CGAAGCCATACCCAATCGAA	55.0	50.0	57.9	58.1	763 bp	0.5	58.5
trnT-UGU-rps4_p2	AGGGAGGGGTCAAACTTCTT	CGAAGCCATACCCAATCGAA	50.0	50.0	58.2	58.1	1386 bp	0.5	58.3
trnT-UGU-rps4_p3	CTCAGAGGTTAGAGCATCGC	AAAATCTGACCGGTTGACCC	55.0	50.0	57.9	58.1	705 bp	0.5	58.2
trnT-UGU-rps4_p4	CTCAGAGGTTAGAGCATCGC	CCGGTTGACCCTTTAGCTTT	55.0	50.0	57.9	58.1	696 bp	0.5	58.2
trnT-UGU-rps4_p5	CTCAGAGGTTAGAGCATCGC	AGCGCGATTGGTTTCTAAGT	55.0	45.0	57.9	57.9	569 bp	0.5	58.2