Hotspot

rpoC1-rpoB

LSC · 25428-25453 · 26 bp

Back to Swartzia

Pi 0.0769

MarkerSeek score 77.90

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 2
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGGCCCAAGCACTTATTTGT	TTGCTCGTTCGAGAACTACG	45.0	50.0	58.0	58.0	192 bp	1.0	80.4
rpoC1-rpoB_p2	TGGCCCAAGCACTTATTTGT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	58.0	57.9	175 bp	1.0	80.0
rpoC1-rpoB_p3	GCTTTGTTACCTCCCCAACT	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.7	58.0	237 bp	1.0	78.0
rpoC1-rpoB_p4	TGGCCCAAGCACTTATTTGT	TTCGATTGCTCGTTCGAGAA	45.0	45.0	58.0	57.7	197 bp	1.0	78.0
rpoC1-rpoB_p5	TGGCCCAAGCACTTATTTGT	TTTCGATTGCTCGTTCGAGA	45.0	45.0	58.0	57.7	198 bp	1.0	78.0