primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCTGGATCAGTCGTGG	GGTTGCGAATACGAGGAGTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.021706	4262	5128	1449-1451	1.000000	87.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCTGGATCAGTCGTGG	AAGACAGGCAAGAAAAGGGG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016812	4262	5128	1546-1553	1.000000	87.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGCTTTTCCCACATCAAGCA	GGTTGCGAATACGAGGAGTT	20	20	45.0	50.0	58.0	58.0	5.6	0.0	0.006322	4262	5128	1708-1711	1.000000	86.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGCTTTTCCCACATCAAGCA	AAGACAGGCAAGAAAAGGGG	20	20	45.0	50.0	58.0	58.0	5.6	0.0	0.001428	4262	5128	1805-1813	1.000000	86.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGCTTTTCCCACATCAAGCA	AAAAGGGGGTAGAGACTGCT	20	20	45.0	50.0	58.0	58.0	5.6	0.0	0.046632	4262	5128	1793-1801	1.000000	86.5
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10525	10839	395-422	1.000000	71.5
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10525	10839	398-425	1.000000	71.2
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10525	10839	401-428	1.000000	70.7
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10525	10839	404-431	1.000000	70.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10525	10839	399-426	1.000000	69.0
rpoC1-rpoB_p1	rpoC1-rpoB	1	TGGCCCAAGCACTTATTTGT	TTGCTCGTTCGAGAACTACG	20	20	45.0	50.0	58.0	58.0	0.0	23.5	0.048496	25428	25453	192-192	1.000000	80.4
rpoC1-rpoB_p2	rpoC1-rpoB	2	TGGCCCAAGCACTTATTTGT	ACGATCTTTGGCTCTGGAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.108847	25428	25453	175-175	1.000000	80.0
rpoC1-rpoB_p3	rpoC1-rpoB	3	GCTTTGTTACCTCCCCAACT	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	57.7	58.0	0.0	23.5	0.328230	25428	25453	237-237	1.000000	78.0
rpoC1-rpoB_p4	rpoC1-rpoB	4	TGGCCCAAGCACTTATTTGT	TTCGATTGCTCGTTCGAGAA	20	20	45.0	45.0	58.0	57.7	0.0	23.5	0.349560	25428	25453	197-197	1.000000	78.0
rpoC1-rpoB_p5	rpoC1-rpoB	5	TGGCCCAAGCACTTATTTGT	TTTCGATTGCTCGTTCGAGA	20	20	45.0	45.0	58.0	57.7	0.0	23.5	0.349560	25428	25453	198-198	1.000000	78.0
psbZ-trnT-UGU_p1	psbZ-trnT-UGU	1	CTTCTCCTGATGGTTGGTCG	AAAATCTGACCGGTTGACCC	20	20	55.0	50.0	58.0	58.1	0.0	9.4	0.110098	39209	39934	1548-1548	0.500000	59.1
psbZ-trnT-UGU_p2	psbZ-trnT-UGU	2	CTTCTCCTGATGGTTGGTCG	CCGGTTGACCCTTTAGCTTT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.110098	39209	39934	1539-1539	0.500000	59.1
psbZ-trnT-UGU_p3	psbZ-trnT-UGU	3	CTTCTCCTGATGGTTGGTCG	AGCGCGATTGGTTTCTAAGT	20	20	55.0	45.0	58.0	57.9	0.0	12.1	0.110498	39209	39934	1412-1412	0.500000	59.1
psbZ-trnT-UGU_p4	psbZ-trnT-UGU	4	CTTCTCCTGATGGTTGGTCG	CTGACCGGTTGACCCTTTAG	20	20	55.0	55.0	58.0	57.9	0.0	9.4	0.110498	39209	39934	1543-1543	0.500000	59.1
psbZ-trnT-UGU_p5	psbZ-trnT-UGU	5	TCGATCCATGCCTCAAAAGG	CCGGTTGACCCTTTAGCTTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137049	39209	39934	1793-1793	0.500000	58.9
trnT-UGU-rps4_p1	trnT-UGU-rps4	1	CTCAGAGGTTAGAGCATCGC	CGAAGCCATACCCAATCGAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.184636	40008	40401	763-763	0.500000	58.5
trnT-UGU-rps4_p2	trnT-UGU-rps4	2	AGGGAGGGGTCAAACTTCTT	CGAAGCCATACCCAATCGAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.217355	40008	40401	1386-1386	0.500000	58.3
trnT-UGU-rps4_p3	trnT-UGU-rps4	3	CTCAGAGGTTAGAGCATCGC	AAAATCTGACCGGTTGACCC	20	20	55.0	50.0	57.9	58.1	0.0	9.4	0.227103	40008	40401	705-705	0.500000	58.2
trnT-UGU-rps4_p4	trnT-UGU-rps4	4	CTCAGAGGTTAGAGCATCGC	CCGGTTGACCCTTTAGCTTT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.227103	40008	40401	696-696	0.500000	58.2
trnT-UGU-rps4_p5	trnT-UGU-rps4	5	CTCAGAGGTTAGAGCATCGC	AGCGCGATTGGTTTCTAAGT	20	20	55.0	45.0	57.9	57.9	0.0	12.1	0.227502	40008	40401	569-569	0.500000	58.2
ycf3_p1	ycf3	1	GGCCCAGAAATCGAGTGAAT	ATCACGTGAGCAGCCATTAG	20	20	50.0	50.0	58.0	58.1	0.0	24.4	0.098735	41523	43475	2567-2567	0.500000	59.2
ycf3_p2	ycf3	2	GGCCCAGAAATCGAGTGAAT	ACACATACAAAGAGACCCGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.138885	41523	43475	2937-2937	0.500000	58.9
ycf3_p3	ycf3	3	GGCCCAGAAATCGAGTGAAT	TGGGGGACATACCGGTATTT	20	20	50.0	50.0	58.0	58.1	0.0	24.7	0.148038	41523	43475	2541-2541	0.500000	58.8
ycf3_p4	ycf3	4	GGCCCAGAAATCGAGTGAAT	CAAAGAGACCCGCCAACATA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.152073	41523	43475	2930-2930	0.500000	58.8
ycf3_p5	ycf3	5	GGCCCAGAAATCGAGTGAAT	CATACAAAGAGACCCGCCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.152073	41523	43475	2934-2934	0.500000	58.8
ycf3-psaA_p1	ycf3-psaA	1	AGCCCTTACCCCTGGTAATT	ATGCCACTCAGCCAAAGAAA	20	20	50.0	45.0	58.0	58.0	6.2	0.0	0.004483	43476	44237	1092-1092	0.500000	60.0
ycf3-psaA_p2	ycf3-psaA	2	CGGGAAACTCTCAAGTACGG	ATGCCACTCAGCCAAAGAAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.008182	43476	44237	1232-1232	0.500000	59.9
ycf3-psaA_p3	ycf3-psaA	3	GAATTGACTGCTGAGCCGTA	ATGCCACTCAGCCAAAGAAA	20	20	50.0	45.0	58.0	58.0	0.3	0.0	0.010497	43476	44237	1257-1257	0.500000	59.9
ycf3-psaA_p4	ycf3-psaA	4	TTAAGGTTTTGAGCAGCGGT	ATGCCACTCAGCCAAAGAAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.024692	43476	44237	1562-1562	0.500000	59.8
ycf3-psaA_p5	ycf3-psaA	5	AGCCCTTACCCCTGGTAATT	CTAGTAGCCCTGCCAAATGG	20	20	50.0	55.0	58.0	58.0	6.2	0.0	0.031259	43476	44237	1439-1439	0.500000	59.7
psaA_p1	psaA	1	CTAATGGCTGCTCACGTGAT	CCCGGAAGTCCACAGAAAAA	20	20	50.0	50.0	58.1	58.0	20.4	0.7	0.078953	44238	46490	2879-2879	0.500000	59.4
psaA_p2	psaA	2	GCGGGTCTCTTTGTATGTGT	CCCGGAAGTCCACAGAAAAA	20	20	50.0	50.0	57.9	58.0	0.0	0.7	0.119103	44238	46490	2509-2509	0.500000	59.0
psaA_p3	psaA	3	GCGGGTCTCTTTGTATGTGT	CCCCCTCGAGTAAAAGCTTC	20	20	50.0	55.0	57.9	58.0	0.0	12.3	0.120351	44238	46490	2643-2643	0.500000	59.0
psaA_p4	psaA	4	GCGGGTCTCTTTGTATGTGT	TCGGTTTCCATTTCGGTTGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124670	44238	46490	2810-2810	0.500000	59.0
psaA_p5	psaA	5	AAATACCGGTATGTCCCCCA	CCCGGAAGTCCACAGAAAAA	20	20	50.0	50.0	58.1	58.0	27.4	0.7	0.128255	44238	46490	2905-2905	0.500000	59.0
psaB_p1	psaB	1	GTACAAGGACGTGCTGTAGG	CTTCCGGTCGAAAAACAACG	20	20	55.0	50.0	58.0	58.0	11.4	0.0	0.021116	46516	48720	2650-2650	0.500000	59.8
psaB_p2	psaB	2	GTACAAGGACGTGCTGTAGG	AAGGGACCCTCCTATGATCG	20	20	55.0	55.0	58.0	58.0	11.4	15.3	0.024735	46516	48720	2814-2814	0.500000	59.8
psaB_p3	psaB	3	GCGTGGTTATTGGCAAGAAC	CTTCCGGTCGAAAAACAACG	20	20	50.0	50.0	58.0	58.0	25.3	0.0	0.036097	46516	48720	2744-2744	0.500000	59.7
psaB_p4	psaB	4	GTACAAGGACGTGCTGTAGG	AAGGTTATGAGCCTTGCGAG	20	20	55.0	50.0	58.0	58.0	11.4	0.0	0.037669	46516	48720	2955-2955	0.500000	59.7
psaB_p5	psaB	5	GCGTGGTTATTGGCAAGAAC	AAGGGACCCTCCTATGATCG	20	20	50.0	55.0	58.0	58.0	25.3	15.3	0.039716	46516	48720	2908-2908	0.500000	59.7
trnG-GCC-trnL-UAA_p1	trnG-GCC-trnL-UAA	1	CGTTGTTTTTCGACCGGAAG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.017909	49626	50924	1920-1920	0.500000	59.9
trnG-GCC-trnL-UAA_p2	trnG-GCC-trnL-UAA	2	CGATCATAGGAGGGTCCCTT	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.0	58.0	13.1	0.0	0.021528	49626	50924	1756-1756	0.500000	59.8
trnG-GCC-trnL-UAA_p3	trnG-GCC-trnL-UAA	3	CGTTGTTTTTCGACCGGAAG	CCGCTCAGATCCATTTGTGA	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.041705	49626	50924	2632-2632	0.500000	59.7
trnG-GCC-trnL-UAA_p4	trnG-GCC-trnL-UAA	4	CGTTGTTTTTCGACCGGAAG	TCCGCTCAGATCCATTTGTG	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.041705	49626	50924	2633-2633	0.500000	59.7
trnG-GCC-trnL-UAA_p5	trnG-GCC-trnL-UAA	5	CTTGAGGTCACGGGTTCAAA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	4.2	0.0	0.042830	49626	50924	1598-1598	0.500000	59.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.539129	54440	55069	773-800	1.000000	82.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.7	57.5	0.0	0.0	0.882901	54440	55069	772-799	1.000000	80.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.7	57.8	0.0	0.0	0.893974	54440	55069	780-807	1.000000	79.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCTCTGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.914922	54440	55069	781-808	1.000000	79.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCTTTGCTAATCGGGGCTAA	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.7	58.7	0.0	0.0	1.025195	54440	55069	761-788	1.000000	78.9
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	AAGCCCCGAACCATAGAGTA	20	20	45.0	50.0	57.9	57.8	5.7	0.0	0.283923	70570	71097	545-627	1.000000	85.3
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	AAAACAAACGCGCTACCAAG	GTACTTACCACTGGTGCCAC	20	20	45.0	55.0	57.9	58.2	5.7	11.3	0.309310	70570	71097	528-610	1.000000	85.2
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CAAAACAAACGCGCTACCAA	AAGCCCCGAACCATAGAGTA	20	20	45.0	50.0	58.2	57.8	5.7	0.0	0.341180	70570	71097	546-628	1.000000	84.8
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAAAACAAACGCGCTACCAA	GTACTTACCACTGGTGCCAC	20	20	45.0	55.0	58.2	58.2	5.7	11.3	0.366568	70570	71097	529-611	1.000000	84.7
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TTGTACCCAAAACAAACGCG	AAGCCCCGAACCATAGAGTA	20	20	45.0	50.0	57.8	57.8	3.5	0.0	0.360337	70570	71097	553-635	1.000000	84.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGCAACAAATACAAACGTTGGA	TCCTAAGAGCAGCGTGTCTA	22	20	36.4	50.0	57.6	57.9	10.3	0.0	2.496132	118556	119378	888-901	1.000000	68.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GTGCAACAAATACAAACGTTGG	TCCTAAGAGCAGCGTGTCTA	22	20	40.9	50.0	57.6	57.9	0.0	0.0	2.554684	118556	119378	889-902	1.000000	68.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGCAACAAATACAAACGTTGGA	AGCAGCGTGTCTACCAATTT	22	20	36.4	45.0	57.6	57.8	10.3	0.0	2.556859	118556	119378	881-894	1.000000	68.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGCAACAAATACAAACGTTGGA	CCTAAGAGCAGCGTGTCTAC	22	20	36.4	55.0	57.6	57.8	10.3	0.0	2.568150	118556	119378	887-900	1.000000	68.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGCAACAAATACAAACGTTGGA	CTAAGAGCAGCGTGTCTACC	22	20	36.4	55.0	57.6	57.8	10.3	0.0	2.568150	118556	119378	886-899	1.000000	68.1