Hotspot

rps16

LSC · 4398-5518 · 1121 bp

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Pi 0.0212

MarkerSeek score 62.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1121
variable_sites: 23
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAAGCCGAGTACTCTACCGT	TCAATTACACAAACTAGAGGAATGT	50.0	32.0	58.2	56.5	1207-1233 bp	1.0	44.1
rps16_p2	AAGCCGAGTACTCTACCGTT	TCAATTACACAAACTAGAGGAATGT	50.0	32.0	58.2	56.5	1206-1232 bp	1.0	44.1
rps16_p3	AAGCCGAGTACTCTACCGTT	TGAATCAATTACACAAACTAGAGGA	50.0	32.0	58.2	56.2	1210-1236 bp	1.0	44.1
rps16_p4	AAAGCCGAGTACTCTACCGT	TCCATCATTGAATCAATTACACA	50.0	30.4	58.2	54.3	1219-1245 bp	1.0	44.0
rps16_p5	AAGCCGAGTACTCTACCGTT	TCCATCATTGAATCAATTACACA	50.0	30.4	58.2	54.3	1218-1244 bp	1.0	44.0