Hotspot

petD

LSC · 74287-75498 · 1212 bp

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Pi 0.00664

MarkerSeek score 50.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1212
variable_sites: 8
pi: 0.00664
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAATTCTATGAAGAGA	CTACTCCCTAGATACCCGCG	36.0	60.0	57.5	58.5	1294-1295 bp	1.0	40.9
petD_p2	AGTTGAAGGGAATTCTATGAAGAGA	ACTACTCCCTAGATACCCGC	36.0	55.0	57.5	57.4	1295-1296 bp	1.0	40.9
petD_p3	GTTGAAGGGAATTCTATGAAGAGA	CTACTCCCTAGATACCCGCG	37.5	60.0	56.2	58.5	1293-1294 bp	1.0	40.9
petD_p4	AGTTGAAGGGAATTCTATGAAGAG	CTACTCCCTAGATACCCGCG	37.5	60.0	56.2	58.5	1294-1295 bp	1.0	40.9
petD_p5	AGTTGAAGGGAATTCTATGAAGAGA	CCTAGATACCCGCGTCTTTT	36.0	50.0	57.5	57.1	1288-1289 bp	1.0	40.9