Hotspot

ndhG

SSC · 116382-116912 · 531 bp

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Pi 0.0113

MarkerSeek score 63.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 6
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TCTTTGGTCTTTACTAATTAGCACT	TCCCGGTTAAATAAATAAGGTCA	32.0	34.8	56.2	55.4	621-622 bp	1.0	40.9
ndhG_p2	TCTTTGGTCTTTACTAATTAGCACT	CCCGGTTAAATAAATAAGGTCATGA	32.0	36.0	56.2	57.2	620-621 bp	1.0	40.9
ndhG_p3	TCTTTGGTCTTTACTAATTAGCACT	TCCCGGTTAAATAAATAAGGTCATG	32.0	36.0	56.2	57.2	621-622 bp	1.0	40.9
ndhG_p4	TCTTTGGTCTTTACTAATTAGCACT	TTCCCGGTTAAATAAATAAGGTCA	32.0	33.3	56.2	56.1	622-623 bp	1.0	40.9
ndhG_p5	TCTTTGGTCTTTACTAATTAGCAC	TCCCGGTTAAATAAATAAGGTCA	33.3	34.8	54.9	55.4	621-622 bp	1.0	40.9