Hotspot

rps15-ycf1

SSC · 121646-121999 · 354 bp

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Pi 0.021

MarkerSeek score 65.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 354
variable_sites: 7
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACGGATCCTCTGCTTTC	TGTATGCGCGATTTCAAATTCA	50.0	36.4	57.6	57.7	435-442 bp	1.0	63.2
rps15-ycf1_p2	CAACGGATCCTCTGCTTTCT	TGTATGCGCGATTTCAAATTCA	50.0	36.4	57.6	57.7	434-441 bp	1.0	63.2
rps15-ycf1_p3	TCAACGGATCCTCTGCTTTC	TGCGCGATTTCAAATTCATT	50.0	35.0	57.6	55.2	431-438 bp	1.0	60.0
rps15-ycf1_p4	CAACGGATCCTCTGCTTTCT	TGCGCGATTTCAAATTCATT	50.0	35.0	57.6	55.2	430-437 bp	1.0	60.0
rps15-ycf1_p5	TCAACGGATCCTCTGCTTTC	ACATATGTATGCGCGATTTCA	50.0	38.1	57.6	56.2	440-447 bp	1.0	59.7