Hotspot

rpl32-trnL

SSC · 111638-112268 · 631 bp

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Pi 0.0211

MarkerSeek score 72.50

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 631
variable_sites: 13
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGAAGGGATATTGGACATCGT	AGCGTGTCTACCGATTTCAC	47.6	50.0	57.2	58.0	743-755 bp	1.0	68.6
rpl32-trnL_p2	GGAAGGGATATTGGACATCGT	CAGCGTGTCTACCGATTTCA	47.6	50.0	57.2	58.0	744-756 bp	1.0	68.6
rpl32-trnL_p3	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	753-765 bp	1.0	67.7
rpl32-trnL_p4	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	752-764 bp	1.0	67.1
rpl32-trnL_p5	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	751-763 bp	1.0	67.1