Hotspot

trnL-ndhB

IRb · 92095-92703 · 609 bp

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Pi 0

MarkerSeek score 63.20

Primer available yes

Label trnL-ndhB

Feature Metadata

label_name: trnL-ndhB
feature_type: igs
region: IRb
length_bp: 609
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ndhB_p1	GTCTCGCGTGTCTACCATTT	ACCCGATTATTGCGATTGCT	50.0	45.0	58.0	58.0	647-685 bp	1.0	83.7
trnL-ndhB_p2	CTCGCGTGTCTACCATTTCA	ACCCGATTATTGCGATTGCT	50.0	45.0	58.0	58.0	645-683 bp	1.0	83.7
trnL-ndhB_p3	GTCTCGCGTGTCTACCATTT	TTGCGATTGCTCAGGATACC	50.0	50.0	58.0	58.0	638-676 bp	1.0	83.6
trnL-ndhB_p4	CTCGCGTGTCTACCATTTCA	TTGCGATTGCTCAGGATACC	50.0	50.0	58.0	58.0	636-674 bp	1.0	83.6
trnL-ndhB_p5	AACCTCCACGCTTTTTAGCA	ACCCGATTATTGCGATTGCT	45.0	45.0	58.0	58.0	678-716 bp	1.0	83.5