Hotspot

petB-petD

LSC · 74090-74286 · 197 bp

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Pi 0.0326

MarkerSeek score 63.80

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 6
pi: 0.0326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	ACCTCTTCTTACGGCCGTAT	TATTTCTGCATGGCCCGATC	50.0	50.0	58.2	58.1	318 bp	1.0	82.1
petB-petD_p2	ACCTCTTCTTACGGCCGTAT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.2	57.7	294 bp	1.0	80.7
petB-petD_p3	TACCTCTTCTTACGGCCGTA	TATTTCTGCATGGCCCGATC	50.0	50.0	57.6	58.1	319 bp	1.0	80.6
petB-petD_p4	TTACCTCTTCTTACGGCCGT	TATTTCTGCATGGCCCGATC	50.0	50.0	58.5	58.1	320 bp	1.0	80.3
petB-petD_p5	CAATCCACTTTGACGCGTTT	TATTTCTGCATGGCCCGATC	45.0	50.0	57.6	58.1	362 bp	1.0	80.2