Hotspot

psaJ-rpl33

LSC · 64645-65101 · 457 bp

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Pi 0.0114

MarkerSeek score 66.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 457
variable_sites: 5
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCGGTTCGTTAGCAGGTTT	AACATCTTTACCCTTCGCCA	45.0	45.0	57.8	57.1	535-553 bp	1.0	74.6
psaJ-rpl33_p2	ATGGTTCGGTTCGTTAGCAG	AACATCTTTACCCTTCGCCA	50.0	45.0	58.3	57.1	539-557 bp	1.0	74.3
psaJ-rpl33_p3	TTCGGTTCGTTAGCAGGTTT	CGAACATCTTTACCCTTCGC	45.0	50.0	57.8	56.9	537-555 bp	1.0	72.9
psaJ-rpl33_p4	TTCGGTTCGTTAGCAGGTTT	ACATCTTTACCCTTCGCCAT	45.0	45.0	57.8	56.9	534-552 bp	1.0	72.8
psaJ-rpl33_p5	ATGGTTCGGTTCGTTAGCAG	CGAACATCTTTACCCTTCGC	50.0	50.0	58.3	56.9	541-559 bp	1.0	72.6