Hotspot

trnP-psaJ

LSC · 64168-64515 · 348 bp

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Pi 0.0203

MarkerSeek score 63.40

Primer available yes

Label trnP-psaJ

Feature Metadata

label_name: trnP-psaJ
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 7
pi: 0.0203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-psaJ_p1	ACCCGTGACATTTTGTACCC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	476-477 bp	1.0	79.1
trnP-psaJ_p2	CCCGTGACATTTTGTACCCA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	475-476 bp	1.0	79.1
trnP-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	487-488 bp	1.0	79.0
trnP-psaJ_p4	CAGGATTTGAACCCGTGACA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	486-487 bp	1.0	79.0
trnP-psaJ_p5	TGACAGGATTTGAACCCGTG	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	489-490 bp	1.0	79.0