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petA-psbJ

LSC · 60690-61556 · 867 bp

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Pi 0.0185

MarkerSeek score 60.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 16
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAAATCTTTTTGGTTCT	GCTGGTATTCTTGTGATCGGT	36.4	47.6	57.2	58.1	1012-1013 bp	1.0	50.7
petA-psbJ_p2	TGGCACAAATCTTTTTGGTTCT	AGCTGGTATTCTTGTGATCGG	36.4	47.6	57.2	57.8	1013-1014 bp	1.0	50.0
petA-psbJ_p3	TGGCACAAATCTTTTTGGTTCT	ACTGGAGCTGGTATTCTTGTG	36.4	47.6	57.2	57.7	1018-1019 bp	1.0	48.8
petA-psbJ_p4	TGGCACAAATCTTTTTGGTTCT	AGGTACTGGAGCTGGTATTCT	36.4	47.6	57.2	57.6	1022-1023 bp	1.0	48.2
petA-psbJ_p5	TGGCACAAATCTTTTTGGTTCTT	GCTGGTATTCTTGTGATCGGT	34.8	47.6	57.9	58.1	1012-1013 bp	1.0	48.0