Hotspot

trnT-trnL

LSC · 45100-45919 · 820 bp

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Pi 0.0134

MarkerSeek score 60.60

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 11
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCAATTCC	55.0	55.0	58.2	58.2	912-919 bp	1.0	78.6
trnT-trnL_p2	GAGTCGAACCGATGACCATC	AGTCCGTAGCGTCTACCAAT	55.0	50.0	58.2	58.2	915-922 bp	1.0	77.9
trnT-trnL_p3	GAGTCGAACCGATGACCATC	TAAGTCCGTAGCGTCTACCA	55.0	50.0	58.2	57.6	917-924 bp	1.0	76.6
trnT-trnL_p4	GAGTCGAACCGATGACCATC	AAGTCCGTAGCGTCTACCAA	55.0	50.0	58.2	58.5	916-923 bp	1.0	76.1
trnT-trnL_p5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.5	58.2	873-880 bp	1.0	75.9