Hotspot

ycf9-trnG

LSC · 34868-35280 · 413 bp

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Pi 0.0266
MarkerSeek score 61.20
Primer available yes
Label ycf9-trnG

Feature Metadata

label_name
ycf9-trnG
feature_type
igs
region
LSC
length_bp
413
variable_sites
11
pi
0.0266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf9-trnG_p1 TGGATTAGTCTTCTTAGTGGGT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 55.9 57.8 506-541 bp 1.0 47.6
ycf9-trnG_p2 AGTCTTCTTAGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 42.9 50.0 54.8 57.8 500-535 bp 1.0 46.8
ycf9-trnG_p3 TTGGATTAGTCTTCTTAGTGGGT CGTCTTCTCCTTGGCAAAGA 39.1 50.0 56.6 57.8 507-542 bp 1.0 45.4
ycf9-trnG_p4 AGTCTTCTTAGTGGGTATCCTT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 55.6 57.8 500-535 bp 1.0 45.3
ycf9-trnG_p5 TCTGGTACATCTTTATGGATTGGA CGTCTTCTCCTTGGCAAAGA 37.5 50.0 57.2 57.8 526-561 bp 1.0 41.7