Hotspot

atpH-atpI

LSC · 12741-13240 · 500 bp

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Pi 0.0101

MarkerSeek score 63.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 5
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	667-671 bp	1.0	80.0
atpH-atpI_p2	TAGAAGCAAGACCAACAGCC	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.8	58.0	643-647 bp	1.0	79.4
atpH-atpI_p3	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	671-675 bp	1.0	79.4
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	644-648 bp	1.0	79.2
atpH-atpI_p5	TAGAAGCAAGACCAACAGCC	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.8	57.9	620-624 bp	1.0	78.6