Hotspot

atpF-atpH

LSC · 12053-12494 · 442 bp

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Pi 0.0253

MarkerSeek score 71.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 11
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CCCGAGGCAGAAGGAAAAAT	50.0	50.0	57.9	58.2	624-631 bp	1.0	80.1
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	553-560 bp	1.0	78.3
atpF-atpH_p3	CCAGTGACCCAAGTAAACGA	CCCGAGGCAGAAGGAAAAAT	50.0	50.0	57.5	58.2	597-604 bp	1.0	76.8
atpF-atpH_p4	GGCCAGTGACCCAAGTAAAC	CCCGAGGCAGAAGGAAAAAT	55.0	50.0	58.8	58.2	599-606 bp	1.0	75.1
atpF-atpH_p5	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.5	57.6	526-533 bp	1.0	75.1