Hotspot

rpl32-trnL-UAG

SSC · 107226-107922 · 697 bp

Back to Suaeda

Pi 0.134
MarkerSeek score 80.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
697
variable_sites
253
pi
0.134
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCGTTAAAAGCTTTT CTCGCACTGCTTCCTAAGAG 45.0 55.0 57.9 58.1 824-966 bp 0.8 82.2
rpl32-trnL-UAG_p2 GGGCAGCGTTAAAAGCTTTT CTCTCGCACTGCTTCCTAAG 45.0 55.0 57.9 58.1 826-968 bp 0.8 82.2
rpl32-trnL-UAG_p3 GGGCAGCGTTAAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 813-955 bp 0.8 81.8
rpl32-trnL-UAG_p4 GGGCAGCGTTAAAAGCTTTT AACCATTGGGATTGGAGAGC 45.0 50.0 57.9 57.9 1406-1538 bp 0.8 81.6
rpl32-trnL-UAG_p5 GGGCAGCGTTAAAAGCTTTT CCGACGTAGCAAATCCTTGA 45.0 50.0 57.9 58.0 1359-1473 bp 0.4 66.7