Hotspot

psbE-petL

LSC · 60492-61348 · 857 bp

Back to Suaeda

Pi 0.0628
MarkerSeek score 81.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
857
variable_sites
148
pi
0.0628
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AAATAACTAGTTAGAGTAGGCAT 45.0 30.4 58.0 52.4 930-1278 bp 1.0 52.0
psbE-petL_p2 CAACCCGCAATGAATAGGGA AAATAACTAGTTAGAGTAGGCAT 50.0 30.4 57.9 52.4 919-1267 bp 1.0 52.0
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC AAATAACTAGTTAGAGTAGGCAT 50.0 30.4 57.8 52.4 842-1190 bp 1.0 52.0
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AAATAACTAGTTAGAGTAGGCAT 55.0 30.4 58.2 52.4 837-1185 bp 1.0 52.0
psbE-petL_p5 AACCCGCAATGAATAGGGAC AAATAACTAGTTAGAGTAGGCAT 50.0 30.4 57.9 52.4 918-1009 bp 0.9 48.0