Hotspot

petA-psbJ

LSC · 58820-59668 · 849 bp

Pi 0.0873

MarkerSeek score 80.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 214
pi: 0.0873
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAAGCCGTCCTTCCAGATAC	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.1	58.0	2295-2393 bp	0.8	82.4
petA-psbJ_p2	GGCGGCCGGTATAGTAAAAA	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1567-1599 bp	0.3	55.3
petA-psbJ_p3	GGCGGCCGGTATAGTAAAAA	ACGACACAATCAAACCCGAA	50.0	45.0	58.0	58.0	1570-1602 bp	0.3	55.3
petA-psbJ_p4	GGCGGCCGGTATAGTAAAAA	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1882-1914 bp	0.3	55.1
petA-psbJ_p5	GGCGGCCGGTATAGTAAAAA	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1804-1836 bp	0.3	54.9